![]() Resonance Raman Spectra of holoHasA WT and holoH83A Mutant Like holoHasA WT, no detectable change was observed after the addition of a 15-fold excess of a KCN solution neither at pH 7.3, where a HS species is dominant, nor at pH 9.4, where two HS species and one LS species are present. Thus, at alkaline pH, holoH83A exists as a mixture of a HS and a LS species, the HS species being different from the one observed at acidic pH. Moreover, a LS species appears, as shown by a new band at 576 nm. The disappearance of the charge transfer band at 630 nm at the advantage of the 610-nm band indicates that the HS species observed at acidic and neutral pH progressively disappears when pH increases for the benefit of a new HS species. When pH increases, bands at 502, 536, and 630 nm decrease, whereas new bands emerge at 490, 543, and 610 nm. This pattern is consistent with the presence of a HS species. At acidic and neutral pH, the spectra exhibit a Soret maximum at 404 nm, Q-bands at 502 nm and 536 nm, and a CT band at 630 nm ( Fig. In contrast to holoHasA WT, the absorbance spectrum of holoH83A is sensitive to pH. Unless otherwise specified, NMR spectra were recorded at 303 K. Spectral widths of 220 ppm in both dimensions with the carriers at 150 ppm for the indirect dimension and at 110 ppm for the direct dimension were used. Direct detected 13C- 13C COSY experiments were acquired at 16.4 T with 2048 × 256 data points, 1024 scans, and a recycle delay of 300 ms for holo-HasA WT and 4096 × 512 data points, 512 scans, and a recycle delay of 250 ms for holoH83A. #Rmn matera seriesSeveral series were performed using 10 and 100 ms as recycle delays with the 13C-carrier frequency at different positions to properly invert and excite all 13C resonances. 13C T 1 values were measured via the inversion recovery sequence. Several experiments were performed with carriers at -200, 150, 567, and 850 ppm to identify signals over wide spectral regions. Onedimensional 13C NMR spectra were acquired at 16.4 T with a 12.4-μs 90° pulse, a spectral width of 500 ppm, and 4096 data points. One-dimensional 15N NMR spectra were acquired at 9.4 T with a 30-μs 90° pulse, a spectral width of 450 ppm, 2048 data points, and a recycle delay of 10 ms. During the recycle delay of 150 or 30 ms, respectively, water suppression was achieved by presaturation. Onedimensional 1H NMR spectra were acquired with 8192 data points either at 16.4 T with a spectral width of 240 ppm or at 11.7 T with a spectral width of 200 ppm. NMR Spectroscopy-NMR experiments were performed on protein samples with concentration ranging from 0.8 to 2.5 m m in 20 m m phosphate buffer adjusted at the requested pH. Glycobiology and Extracellular Matrices.The interior is in the shape of a Latin cross and has an ancient nativity scene that dates back to the 1500s inspired by the caves in the Sassi di Matera area, the Last Judgment painted by Rinaldo da Taranto, the Renaissance chapel of the Annunziata, and the 13th-century fresco of Our Lady of Bruna. There are two other entrances on the sides - the square door with a bas-relief of Abraham, and the other, the lions’ door with two stone-carved lions. Paul sculpted by Altobello Persio in 1540 can be seen. della Bruna and on the sides the statues of St. On the architrave of the main door there is a niche containing a statue of Maria SS. Located in the city's historic center, the 4-story square-based bell tower catches the eye with its facade decorations and Romanesque rose window featuring a 16-spoke wheel of fortune topped off by a statue of the Archangel Michael. The Cathedral with its Apulian Romanesque style still clearly visible, is dedicated to Our Lady of Bruna and St. ![]()
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